Development of the an Immumoassay for Simultaneous Analysis of Vardenafil and Its Analogues

Abstract:

Vardenafil is a phosphodiesterase type-5(PDE-5) inhibitor for the treatment of erectile dysfunction.Undeclared vardenafil and the related analogues adulterated in herbal commodities are a threat to human health.The anti-vardenafil McAb of 4B9 was the specific to vardenafil and its analogues,based on this result,an indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) was developed.The limit of detection of vardenafil was 5.0 ng/mL-1,the calibration curve was linear from 5.0 to 40 ng/mL-1(R2=0.952) with an IC50 value of 18.2 ng/mL-1.In the extracts of 20 Chinese traditional drugs,the detection capability(CCβ) of vardenafil was 0.08 mg/g-1,the recoveries were 76-116% and the coefficients of variation(C.V.) were 9.7%-16.2%.