Preliminary Studies on Enzymatic Degumming for Apocynum venetum L.

Abstract:

This manuscript aims to attain the optimum degumming conditions for Apocynum venetum L by a new strain-Acinetobacter junii FM208850,the response surface methodology designed by Box-Behnken was applied based on the single factor experiment.According to the regression analysis,the optimal conditions were determined and listed as follows: Na2CO3 was 0.2 g/100mL H2O to make initial pH 9.5,pectinase amount of 8.38 mL(161.3 U/mL),bath ratio of 1∶16,temperature of 45 ℃,time of 10 h;The actual residual gum content was 7.937% under the optimum conditions.