Studies on Detection of Staphylococus aureus in Foods by Multiplex PCR

Abstract:

In this manuscript,a multiplex PCR assay for detection of S.aureus was developed by designing three sets of primers that specifically amplify segments of the nuc、clfA and SmaI genes.Analysis of ten S.aureus strains and forty-six non-S.aureus strains demonstrated that this PCR system was specific.The detection limit of the PCR was 1.1973 ng with genomic DNA and 1.2 cfu/g in artificially contaminated bread after enrichment at 37 ℃ for 6 h.Forty-three foods were detected to confirm the accuracy of this method.Among of them,three were found to be positive,and two were consistent with that of the international standard method,and one was through verification by sequenced.The result proven that the specific multiplex PCR assay can be used for detection of S.aureus in foods.