Abstract:In order to utilize polysaccharides resources in Hyriopsis cumingii,polysaccharides from Hyriopsis cumingii(HCPS) was extracted by using methods of hot water lixiviation,ethanol precipitation,Sevag’s deproteination and ethanol precipitation again,the extraction yield of crude HCPS was about 3.5%.The crude HCPS was separated firstly through DEAE-cellulose 52 chromatography column and further purified through chromatography column of Sephadex G-100.Three purified fractions,named as HCPS-1,HCPS-2 and HCPS-3,were obtained.The recovery rates of HCPS-1,HCPS-2 and HCPS-3 was 26.9%,30.2% and 3.9% respectively.Preliminary feature of crude HCPS,HCPS-1,HCPS-2 and HCPS-3,including polysaccharides content,protein content,sulfuric radical content,relative viscosity,monosaccharide composition,relative molecular weight and FTIR spectrum,were determined.Results showed that HCPS-3 had more sulfuric acid radicals and protein than HCPS-1 and HCPS-2.The monosaccharide composition of HCPS-3 was more complicated than that of HCPS-1 or HCPS-2.HCPS-3 was quite different from HCPS-1 or HCPS-2.
