Improvement of Extracellular Laccase Production by Paecilomyces sp. S152

Abstract:

The culture medium containing maltose(20 g/L),bean peptone(10 g/L) and yeast extract(1 g/L) was found to favor laccase production by Paecilomyces sp.S152 with an enzyme activity of 5064 U/L.The intra- and extra- cellular location of laccases were detected in the cells of Paecilomyces sp.S152.To improve cell permeability,Tween 80(1.5 g/L) was added to culture broth and an enhanced extracellular laccase activity was detected with the shortened fermentation period(6 days) and improved(946 U/(L·d).