Isolation and Identification of Cholesterol-Lowering Lactic Acid Bacteria from Spinach

Abstract:

Total 33 strains of Lactic acid bacteria were isolsted from spinach,using four different kinds of media.All strains could reduce cholesterol from media by sulfate-phosphate-ferric methods,and the percentage of cholesterol-removal rate ranged from 4.82%to 47.58%.There are 9 strains exhibited the percentage of cholesterol-removal above 40%,among them,the cholesterol-removal percentage of strain MB65 achieved at 47.58%.Based on morphological, physiological,biochemistry test and 16S rDNA sequencing analysis,strain MB65 was identified as Lactobacillus casei.