Abstract:β-Lactam antibiotics residue trace detection has proved to be an important safety problem,due to its relationship of animal derived food safety and international trade.Biosensor which would rapidly and automatically detectingβ-Lactam antibiotics in convenient,has attracted more and more researchers.Based on the different biological reaction,it can be devided into three classes:enzymic,penicillin binding proteins(PBPs) and immune.Firstly,enzymic biosensor has been took into practice,but their capacities were not enough to residue detection.PBPs were a kind ofβ-Lactam antibiotics sensitive bacteria functional protein,which needs to be determined, characterized,and produced through advanced biotechnology.Comparative advantage of immunosensors was its low detection limit and systematic development.Recently,more research concerned with the immunosensors and studied on the new transducer,nano-technology and multi-residue automatically detection.New achievements and methodologies of computer, molecular biology and bioinformatics had been used to design and screen bio-recognition elements for biosensors development,as well as to investigate the biosensing process.It hopes that biosensor practicability would be improved though new biological research methods and findings.
