D-lactic Acid Production of High-Yielding Strains of Breeding

Abstract:

In the paper,the Bacillus coagulans JD-063 was mutagenized using UV,diethyl sulfate,cobalt 60γ-ray and microwave;Mutant strains were carried out fermentation test and genetic atability test.Finaly,we selected a strain JD-76D which had some notable features.Using the mutant strain,the amount of produced acid was increased to 145 g/L from 61 g/L using starting strain,the purity of D-lactic acid reached to 98.7% which was more than 97.5% of original strain.