Screening,Identification and Primary Optimizing of a Strain Producing γ-Aminobutyric Acid from L-Glutamic Acid

Abstract:

A strain with capablity of producing γ-Aminobutyric acid from L-glutamic acid was isolated from variety of fermented food and different natutal environment samples,and the conditions of transformation were optimized.Process and Method: Isolation and purification method of Lactic acid bacteria was used to screen the strain yielding γ-amino butyric acid,and its morphology analysis,physiological and biochemical properties and phylogenetic analysis based on the 16S rDNA sequence were identified.And then the conditions of transformation were optimized for the Lactic acid bacteria separately.Based on Systems Manual of Determinative Common Bacteriology,the bacteria was preliminarily identified Lactobacillus plantarum.The better transformation conditions as follows: cell age 36 h,buffer initial pH 4.8,buffer concentration 0.2 mol/L;The content of GABA was over 34.26 g/L and transformation efficient was up to 97.81% in the optimum transformation conditions.The strain can be used to produce γ-amino butyric acid and has a higher converting capability of GABA.