The Study of Purification of Taxol from Endophytic Fungi Fermentation Broth by D4020 Macroporous Resin

Abstract:

Absorption on D4020 macroporous resin was studied as a method to purify the taxol from endophytic fungi fermentation broth.The optimum conditions for absorption and desorption of taxol was determined and listed as follows: The adsorbed temperature at room temperature,the sample was dissolved in 50 % methanol,and added on a column of the resin at the rate of 1.5 mL/min,then it was washed with 20% ethanol,and was eluted with 7 BV 80% ethanol at 0.5 mL/min speed.Under the optimum conditions,the recovery rate achieved at 90%,so this resin can be used for separation and concentration of taxol.The static absorption dynamic curve and isotherm of taxol on D4020 resin showed that D4020 macroreticular resin was suitable for absorbing and separating taxol.