Study on the Separation of Lycopene Isomers Via Cosmosil Cholester-HPLC System

Abstract:

Lycopene is a ploy-unsaturated carotene with 11 conjugated and 2 non-conjugated carbon-carbon double bonds.Because of its 13 double bonds,lycopene is susceptible to isomerize and isomerization may influence its bioactivity.In this paper,the authors studied the methodology of lycopene isomers detectation via COSMOSIL Cholester-HPLC system.The optimized conditions were as follows:a mixture of tetrahydrofuran and acetonitrile(10∶90,v/v) was used as mobile phase and its flow rate was 1mL/min,the injection volume was 20 μL,and the separating was monitored at 472nm.Confirmatory test was done and the result showed that this HPLC system was effective in lycopene isomers detection with ideal resolution and fairly good repeatability.