Production and Characterization of Extracellular β-glycoside Hydrolase from Trichderma Asperellum W03

Abstract:

The strain of Trichderma asperellum W03 was isolated from the peat and exhibited the potential to produce xylanase,CMCase and chitinase under the submerged culture.Those three kinds enzymes achived at a highest activity at the conditions: pH 4.5,temperature 40 ℃-45 ℃ and acidic condition.Moreover,the optimum nutrient and culture condistions were determined and listed as follows: 200.00 potato,2.0 sodium carboxymethyl cellulose,5.0 glucose,1.0 KNO3,1.0 peptone,1.0 KH2PO4,0.5 MgSO4·7H2O and pH 6.0.with the optimum conditions,xylanase,CMCase and chitinase reached at 87u/mL,58 u/mL and 30 u/mL,respectively,after 72 h with 33 ℃ and 180 r/min.