Time-Temperature Indicator based on Thermostable Alpha-Amylase

Abstract:

Time-temperature history is important for controling sterilization process in food industry.Time Temperature Indicator(TTI) can be used to indicate the temperature history inside food.Capsule embedding technology was used to build TTI,in which thermostable alpha-amylase was used as indicactor.Kinetics for thermal inactivation of indicator were built and validated with konjac glucomannan as a carrier.The validation experiment was carried out by fluidization solid food ultra high temperature sterilization technology.Statistical analysis showed that: thermostable alpha-amylase give first-order inactivation kinetics,there is no significantly differences between experimental results and model theoretical results,TTI can be used for studing kinetics of enzyme inactivation,TTI with thermostable alpha-amylase as indicator can be used for time-temperature indicating in fluidization solid food ultra high temperature sterilization process.