The Disruption of URA3 Gene of Yarrowia lipolytica

Abstract:

In order to get high-yield γ-decalactone and genetic markers,an auxotrophic Yarrowia lipolytica strain was isolated by genetic modification.In this study,URA3,a key gene for uracil synthetase of Yarrowia lipolytica,was disrupted using the method of gene homologous recombination,then transformants were screened by uracil auxotrophic medium(SD-URA) with the combination of 5-FOA and uracil.The results showed that the uracil auxotrophic strain was able to grow at the SD-URA medium containing 5-FOA and uracil while the wild-type strains do not grown.Therefore,an easy and efficient method for obtaining auxotrophic Yarrowia lipolytica strain was established in this study.