Abstract:In this study,two strains,TC-3 and TC-2,identified as Bacillus sp.and Bacillus subtilis based on the biological characteristics and 16S rDNA sequence analysis,was screened from 25 isolates of endophytic bacteria from wild Taxus chinensis in Qinba Mountain.Those strain exhibited the ability in producing inhibitory activity substance,Without the effect of organic acid and hydrogen peroxide,supernatant of the liquid culture of TC-3 and TC-2 showed significant inhibitory activity against gram-positive bacteria,including Staphylococcus aureus,Bacillus subtilis and gram-negative bacteria such as Escherichia coli.Inhibition of some kind of epiphyte was also found.After treatment with trypsin,pepsin and proteinase K,the culture’s inhibitory activity decreased slightly,this showed that it is non-protein ingredients or antibacterial effect of the non-single material.This indicated it belongs to a kind of bacteriocin-like substance.The inhibitory substance,showed good heating stability,high activity at low pH,low sensitivity to proteinases and a broad antibacterial spectrum.
