Bioinformatics Analysis on the Sequence of Lipase Gene from Penicillium cyclopium

Abstract:

The gene encoding alkaline lipase(Lip I) is cloned from Penicillium cyclopium PG37 and the genetic information is analyzed by bioinformatic tool.Sequence analysis showed that Lip I DNA is 1480 bp long with no repeat sequence and five introns,and there is a TATA box and several transcription factor binding sites in the 5’ end.Lip I cDNA is 1128 bp long with an open reading frame containing 858 bp.It also has transcription start site,5’ and 3’ non-coding region.TGC,GAC,TTC,CAC,AAG,AAC and TAC codons are used at highest frequency in Lip I gene.Comparing codon usage frequency of the Lip I with Pichia pastoris genome’ showed that the ratios of 21 codon usage frequency are different widely.