Abstract:In this study,a method for determining the content of oligosaccharides,including sucrose,raffinose,stachyose in tobacco casing materials by high performance liquid chromatography(HPLC) was developed.Separation and quantification were achieved by Waters Sugar Pak1(6.5 mm 300 mm,5 μm) with 1.0 mL/min pure water as the mobile phase,column temperature at 85℃ and Refractive Index Detector.The quantified analysis of external standard calibration curves was used.There were good linear correlations between the concentrations of three sugars and their chromatographic peak areas and the correlation coefficients were 0.9991-0.9997.The recoveries were 96.9%-103.5%,the RSD of the method was less than 4.7%.And the detection limits were 3.5-4.0 g/mL.This method is simple,rapid and accurate,and has been applied for eh quantitative analysis of sucrose,raffinose,stachyose in tobacco casing materials.
