Antioxidant Activity and Purification of Pollock Peptides

Abstract:

The antioxidant activities of Pollock peptides were studied at first in this manuscript and demonstrated he Pollock peptides had strong scavenging hydroxyl free radical activity,and scavenging 1,diphenyl-2-picrylhydrazyl(DPPH) activity,and reducing activity.In order to obtain high homogenous antipeptide,the Pollock peptides were purified by ultraffltrations,gel filtration,anion-exchange chromatography(Q-FF),and reversed phase high performance liquid chromatography(RP-HPLC) in turn.These results showed that Pollock peptides separated on Q-FF column and three components(B1,B2,B3) were obtained.B2 was identified by RP-HPLC to be homogenous.