Studies on Fermentation Conditions for Production of Neokestose by Xanthophyllomyces dendrorhous

Abstract:

In order to further improve the titer and yield of neokestose production by Xanthophyllomyces dendrorhous,the medium compositions and environmental conditions for neokestose production were optimum by the single factor experiment and the orthogonal experiments,and listed as follows:sucrose 110 g/L,dried corn steep liquor powder 9 g/L,pH 6.5,rotation speed of shaker 220 r/min and inoculation size10% in 25 mL of culture medium in the 250 mL flasks at 18 ℃ for 24 h.The concentration of neokestose by Xanthophyllomyces dendrorhous 269 was achieved at 73.2 g/L,the yield of neokestose on sucrose reached at 66.5%,which was 1.87 times than that of the control.