Abstract:Microwave-assisted extraction (MAE) was applied to the polysaccharides extraction from the dried root,stem and leaf of Actinidia Chinensis.Three key operation parameters,including the extraction time,the power of microwave and the ratio of material to solvent,were optimized with uniform experimental design method.The optimal conditions for extracting three raw materials were determined by the regression equations.Considering the efficiency and the feasibility of experiment,the actual operating conditions were 0.016 9 of the ratio of material to solvent,24 min of the extraction time and 385 W of the microwave power in the extraction of root;0.0170 of the ratio of material to solvent,23.8 min of the extraction time and 539 w of the microwave power in the extraction of stem;and 0.016 1 of the ratio of material to solvent,23.5 min of the extraction time and 539 W of the microwave power in the extraction of leaf,respectively.The extracted yields of polysaccharides in the actual optimal conditions were 7.0%,3.7%和3.2%.The deviation between the values from experiments and calculation from root and leaf were both below 5%,which demonstrated that the regression equation could predict the yield of polysaccharide in the given conditions well.
