Lipase-catalyzed Synthesis of L-ascorbyl Stearate in Non-aqueous Phase

Abstract:

In this manuscript,the technical conditions of Novozym 435 lipase-catalyzed synthesis of L-ascorbyl stearate (L-AS) in non-aqueous medium were determined with 20% lipase and 1:2 L-ascorbic acid to stearic acid (molar ratio).The optimum reaction conditions listed as follows:30ml t-butanol with 6.82mmol L-ascorbic acid,13.64mmol stearic acid,0.24g lipase and 3.0g 4A molecular sieves,shaker speed 150r/min,the reaction carried out at 55℃ water bath with 48h.Through the separation and purification,the isolated product was finally identified as L-ascorbyl stearate by infrared (IR) and mass spectrometry (MS) detection,the highest yield reached 64.7%.Lipase reused five times,the lowest yield was 61%.