Supercritical CO2 Conditions of CoQ10 Biotransformation from Solanesol by Schizosaccharomyces prombe

Abstract:

Supercritical CO2(scCO2) as non-aqueous media could well dissolve substrates and products of coenzyme Q10(CoQ10) biotransformation reaction from solanesol,but it also had effects on microbial activity,so appropriate scCO2 conditions could improve CoQ10 yield.The effects of scCO2 on the catalytic activity of Schizosaccharomyces prombe(S.promb) and CoQ10 yield in different conditions were studied.The appropriate scCO2 conditions which could significantly promote the production of CoQ10 and could have little negative effects on the cells survival was: triple of cells’ wet weight of culture medium was added into the reaction system.,kept the pressure at 10.5 MPa for 16 h,the production of CoQ10 is 55.71 μg/mL,improved 97.5%.The results showed that optimization of scCO2 reaction system conditions could significantly improve the production of CoQ10.