Application of rpsL Mutation Assay for Mutagenicity with Acrylamide

Abstract:

In this study,rpsL mutation assay was use as model to determine the mutagenicity induced by acrylamide.E.coli MF101 carried with pMOL21 was treated with different concentrations of acrylamide(0.05,0.10,0.25,0.50,1.00 mg/mL,respectively).The results showed that with the increasing of the acrylamide concentration,rpsL mutation frequency significantly increases.Furthermore,a dosage-dependent relationship was detected.The results present here strong suggested that the rpsL mutation assay may be as a potential tool for investigating food and drug safety.