A Protocol for Screening of ε-Polylysine Producing Strains

Abstract:

A novel ε-polylysine produce strain screen protocol was developed in this study based on the chemical characteristics of ε-polylysine.Through this three-steps protocol,42 strains has been identified as lysine polymer producer,then those polymer structure were analysized by chemical method,by which confirms that the polymer is linked by ε-NH2 and α-COOH.Among of them,one strain,0.8 g/L ε-polylysine was found in fermentation broth,was identified as Streptomyces padanus by the combination results of physiological and biochemical characteristics,and 16s rDNA sequence.