Enhancement of Glutathione Synthesis by Improving ATP-Regenerating Efficiency in the Coupled System

Abstract:

A coupled system used for the biosynthesis of glutathione(GSH) was constructed with Escherichia coli Δadd/ade(pBV03) and Saccharomyces cerevisiae WSH2.On one hand,the irreversible transformation from adenosine(Ado) to hypoxathine(Hx) in E.coli Δadd/ade(pBV03) was completely blocked by the disruption of adenosine deaminase(ADA) and adenine deaminase(ADE).On the other hand,the activity of ADE in S.cerevisiae WSH2 decreased greatly when it was cultured in proline minimal medium.Therefore,the transformation from Ado into Hx decreased significantly,and more Ado was used to regenerate ATP by S.cerevisiae WSH2.At last,ATP-regenerating efficiency was improved,and GSH production reached 13.68 mmol/L,which was 5.14 fold of the control.