Optimization and application of ATP Bioluminescence System in the Rapid Evaluation of Microwave Sterilization

Abstract:

In this manuscript,the extraction method of microorganism ATP used in bioluminescence techniques were studied.According to compare the extract results of different extractants,it was found that that benzalkonium bromide(BAB) is the best extractant to extract all of ATP microorganism cells.The extraction conditions including the extractant concentration and the extaction time were determined and listed as follows: 0.05 % BAB mixing with bacteria cells for 3-4 min.The finding was applied to rapidly evaluate the effect of microwave sterilization coupling with bioluminescent technology and the reliable results can be obtained within a short time.According to the sterilization effect of different microwave power and sterilization time,we found that E.Coli ATCC25922 can be completely exterminated irrediated by 2 450 MHz 650 w microwave for 60 s but 70 s for Staphylococcus aureus ATCC25923.When treated with 2450 MHz microwave all for 60 s,the power for compelte extermination of E.Coli ATCC25922 is 650 w but 700 s for Staphylococcus aureus ATC25923.