Optimization of Nutrient and Environmental Conditions for Acetoin Production from Bacillus licheniformis

Abstract:

The nutritional and environmental conditions for acetoin production from Bacillus licheniformis in flasks were studied in this manuscript.Firstly,the environmental conditions,such as pH value,culture temperature,seed age,volume and inoculation ratio,and nutritional conditions,like carbon and nitrogen sources were carefully investigated.Based on the results of the single factor experiment,the orthogonal experiment was carried to get the optimum medium composition.Then fed-batch fermentation was performed with glucose feeding.The yield of acetoin achieved at 20 g/L in fed-batch fermentation with glucose feeding.