Mutant and Optimization of Androstenone Translation Strain Mycobacterium sp. SH5

Abstract:

Mycobacterium sp.SH5 was a potential strain for degrading phytosterol.After mutation by UV and NTG,a mutant with 9α-hydroxylase defective was selected and named as Mycobacterium sp.SH5-52.On the basis of mutation,the nutritional and environmental conditions of Mycobacterium sp.SH5-52 were investigated and optimized.With the optimum conditions,Mycobacterium sp.SH5-52 could degrade 0.6% substance phytosterol to Androst-4-end-3,17-dione(AD) and Androst-1,4-end-3,17-dione(ADD) with the average conversion ratio significantly improved from 37.2% to 47.6%.