Function and Phenotype of Mitochondrial NADH Kinase in Saccharomyces cerevisiae

Abstract:

NADH kinase is the critical pathway for NADP(H) biosynthesis in yeast.In Saccharomyces cerevisiae,mitochondrial NADPH is mainly supplied by a mitochondrial NADH kinase encoded by POS5 gene.However,a mitochondrial NADP+-specific isocitrate dehydrogenase encoded by IDP1 gene could also supply mitochondrial NADPH.Here,through the phenotypic study of POS5 single mutant,IDP1 single mutant,POS5IDP1 double mutant,including their growth ability,temperature sensitivity,non-fermentable carbon sources usage ability,amino acid synthetic ability and anti-oxidation,supplying mode of mitochondrial NADPH and function of NADH kinase in mitochondria were investigated.