Development of a Discontinuous Percoll Gradient Centrifugation Process for Taste bud Cells Purification

Abstract:

In this manuscript,the taste bud cells of fungiform papillae of ICR mice were select as research model and Percoll gradient centrifugation was used as purify method.The methods of trypan blue staining and immunohistochemistry staining were performed to compare the viability and purity of taste bud cells of before and after purification respectively.Taste bud cells were further identified by transmission electron microscopy.Results showed that taste bud cells,which were purified by discontinuous Percoll gradient centrifugation,were remarkably separated and mainly located interface between 20% and 40% Percoll working solution;the purity of taste bud cells were distinctly improved from less than 10% to more than 65%(p<0.01) by this treatment;the viability of taste bud cells were up to 94% after purification by trypan blue staining and the results of transmission electron microscopy further confirmed taste bud cells from ultrastructure.