Fluoroimmunoassay for Human IgG Using Ru（bpy）3Cl2-doped Fluorescent Silica Nanoparticles as Labels

Abstract:

Fluorescent-labeled molecules have been widely used in biological detection and diagnosis.In this manuscritpt,the Ru(bpy)32+-doped silica nanoparticles were firstly prepared with a modified W/O method.Human IgG(hIgG) was measured based on the specific interaction between hIgG and functionalized fluorescent core-shell nanoparticle-labeled gost anti-hIgG.The calibration graph for hIgG was linear over the range 1-100 ng/mL with a detection limit of 0.3 ng/mL.From 11 measurements,the R.S.D.is 2.2% for samples of 30 ng/ mL of hIgG.The results demonstrated that the method have a potential advantages of sensitivity,simplicity and good reproducibility for the determination of hIgG,and is applicable to the determination of hIgG in serum samples.