益生菌-维生素D-肌醇对小鼠妊娠期糖尿病的协同改善作用

Synergistic Effects of Probiotic-Vitamin D-Inositol Combination Intervention on Gestational Diabetes Mellitus on Mice

摘要:

多种膳食因子包括益生菌、维生素D、肌醇对妊娠期糖尿病（gestational diabetes mellitus，GDM）具有缓解作用，但是关于膳食因子复配干预GDM的研究则较少报道。作者将膳食因子进行复配，通过调节多种代谢途径，以应对与GDM相关的复杂代谢紊乱。以高脂饮食+链脲佐菌素（streptozotocin，STZ）诱导形成GDM小鼠模型，益生菌的剂量为5×10⁸ CFU/d，维生素D的剂量为5 μg/（kg·d），肌醇的剂量为100 mg/（kg·d）。结果显示，相比于单独的益生菌干预或两两复配干预，3种膳食因子复配调节小鼠肠道菌群及缓解炎症、氧化应激、内质网应激、肝脏糖脂代谢紊乱、胰岛损伤的作用更加显著，并能够改善血糖与胰岛素稳态。3种膳食因子通过共同调控多种代谢途径，从而发挥协同增效的作用，实现了更佳的健康效益。

Abstract:

Various dietary factors, including probiotics, vitamin D, and inositol, have been shown to alleviate gestational diabetes mellitus (GDM). However, research on the combined intervention of these dietary factors for GDM remains limited. The author aimed to combine these dietary factors and modulate various metabolic pathways to address the complex metabolic disturbances associated with GDM. A GDM mouse model was induced by streptozotocin (STZ) combined with a high-fat diet. The dosage was 5×10⁸ CFU/d for probiotics, 5 μg/(kg·d) for vitamin D, and 100 mg/(kg·d) for inositol. The results indicated that, compared to individual probiotic interventions or pairwise combinations, the three-component dietary intervention more effectively regulated the gut microbiome, alleviated inflammation, oxidative stress, endoplasmic reticulum stress, and disturbances in hepatic glucose and lipid metabolism, as well as pancreatic damage, and improved glucose and insulin homeostasis. This supports the hypothesis that the synergistic effects of these three dietary factors, through their joint regulation of multiple metabolic pathways, can lead to improved health outcomes and greater economic benefits.