产共轭亚油酸Lactiplantibacillus plantarum ZFM804的筛选及益生特性评价

Screening and Evaluating the Probiotic Properties of Lactiplantibacillus plantarum ZFM804 Producing Conjugated Linoleic Acid

摘要:

作者以发酵食品中分离的8株乳酸菌为研究对象，采用紫外分光光度法筛选出产共轭亚油酸（CLA）的菌株，进一步对其体外益生特性进行评价。结果表明，Pediococcus acidilactici ZFM801、Leuconostoc mesenteroides ZFM803、Lactiplantibacillus plantarum ZFM804、Lactiplanti-bacillus plantarum ZFM805、Lactiplantibacillus plantarum ZFM806、Weissella cibaria ZFM810具有产CLA的能力，其中Lactiplantibacillus plantarum ZFM804产CLA能力最高（质量浓度达21.7 mg/L），且CLA异构体分别为c9，t11-CLA和t10，c12-CLA。益生特性评价结果表明，Lactiplantibacillus plantarum ZFM804对3种致病菌的共凝聚率均高达25%以上。耐受性结果表明，Lactiplantibacillus plantarum ZFM804可耐受质量分数4%的NaCl和质量分数0.1%的胆盐；同时Lactiplantibacillus plantarum ZFM804在模拟胃肠液环境下同样表现出较高的耐受性，且pH 4.5时存活率高达60%以上；其次Lactiplantibacillus plantarum ZFM804对藤黄微球菌、金黄色葡萄球菌具有显著的抑制作用。综上，<Lactiplantibacillus plantarum ZFM804具有高产CLA的潜能和良好的益生特性。

Abstract:

In this study, 8 strains of lactic acid bacteria isolated from fermented foods were used to screen the strains producing conjugated linoleic acid (CLA) by ultraviolet spectrophotometry, and further evaluation of in vitro probiotic properties were conducted. The results showed that Pediococcus acidilactici ZFM801, Leuconostoc mesenteroides ZFM803, Lactiplantibacillus plantarum ZFM804, Lactiplanti-bacillus plantarum ZFM805, Lactiplantibacillus plantarum ZFM806, and Weissella cibaria ZFM810, had the ability to produce CLA, among which Lactiplantibacillus plantarum ZFM804 exhibited the highest CLA production capacity (mass concentration up to 21.7 mg/L) and consisted of c9,t11-CLA and t10,c12-CLA isomers. The results of prebiotic properties revealed that Lactiplantibacillus plantarum ZFM804 exhibited a high co-coagulation rate of 25% against three pathogenic bacteria. The tolerability study showed that Lactiplantibacillus plantarum ZFM804 could tolerate 4% mass fraction of NaCl and 0.1% mass fraction of bile salt, and Lactiplantibacillus plantarum ZFM804 also showed high tolerance under a simulate gastrointestinal fluid environment, with a survival rate over 60% at pH 4.5. Moreover, Lactiplantibacillus plantarum ZFM804 demonstrated a significant inhibitory effect on Micrococcus luteus and Staphylococcus aureus. In summary, Lactiplantibacillus plantarum ZFM804 possesses the potential for high CLA production and exhibits excellent prebiotic properties.