不同贮藏条件下芹菜可溶性糖质量分数及相关基因变化分析

Analysis of Soluble Sugar Mass Fraction and Related Genes Changes in Post-Harvest Celery

摘要:

芹菜（Apium graveolens L.）是伞形科二年生叶菜类蔬菜，可溶性糖是叶菜类蔬菜的重要营养成分之一。作者选取‘四季小香芹’和‘六合黄心芹’为试材，测定其在室温（25 ℃）、低温（4 ℃）和室温黑暗（25 ℃）条件下贮藏0、6、24、30、48、54 h的可溶性糖质量分数，利用实时荧光定量PCR技术检测可溶性糖代谢相关基因（AgIVR1、AgSPP、AgSPS、AgSS1 和AgUDPG ）的表达特性。结果显示：3种贮藏条件下芹菜可溶性糖质量分数存在品种和组织差异性，‘四季小香芹’可溶性糖质量分数高于‘六合黄心芹’；叶柄可溶性糖质量分数高于叶片；不同贮藏条件下可溶性糖质量分数无明显变化规律。低温贮藏至54 h时，2种芹菜AgSS1 基因的表达量均显著上升；不同贮藏条件下芹菜的AgSPS 基因表达量整体都呈下降趋势；不同基因之间可能通过互作共同调节芹菜贮藏过程中可溶性糖质量分数的变化，可溶性糖积累量与相关基因的变化有关。

Abstract:

Celery(Apium graveolens L.), a leaf vegetable, is a biennial herb in Apiaceae. Soluble sugar is an important nutritional component of leafy vegetable crops. In this study, two celery cultivars of ‘Sijixiaoxiangqin’ and ‘Liuhehuangxinqin’ were selected as samples. The soluble sugar content was determined and analyzed after storage at room temperature (25 ℃), low temperature (4 ℃) and room temperature in the dark (25 ℃) for 0, 6, 24, 30, 48 and 54 h. The expression characteristics of soluble sugar metabolism-related genes (AgIVR1, AgSPP, AgSPS, AgSS1 and AgUDPG ) were detected by real-time quantitative PCR technology. The results showed that there were varietal and tissue differences in the soluble sugar content of celery under the three storage conditions. The soluble sugar content in ‘Sijixiaoxiangqin’ was higher than that in ‘Liuhehuangxinqin’, and the soluble sugar content in the petioles was higher than that in the leaves. The soluble sugar content had no obvious change under different storage conditions. After storage at low temperature for 54 h, the expression levels of AgSS1 in both celery cultivars increased significantly, and the overall expression level of AgSPS in celery exhibited a downward trend under different storage conditions. Diverse genes might co-regulate the change in soluble sugar content through interaction during celery storage. The accumulation of soluble sugar was correlated with the changes in related genes.