基于多克隆抗体的糠氨酸ic-ELISA检测方法的建立

Development of ic-ELISA for Furosine Detection Based on Polyclonal Antibodies

摘要:

采用甲醛法制备了糠氨酸完全抗原，通过交叉验证确保了多克隆抗体的特异性，从而建立了基于该抗体的糠氨酸间接竞争酶联免疫吸附测定（indirect competitive enzyme-linked immunosorbent assay, ic-ELISA）检测方法。结果显示，建立的ic-ELISA检测方法有效检测范围为12.20~2 143.42 ng/mL，最低检测限为2.69 ng/mL。该方法操作简便、迅速，成本低廉，同时具备高灵敏度和优异特异性，为高效检测食品中糠氨酸质量浓度提供了新的选择，具有一定实际应用价值。

Abstract:

A complete furosine complete antigen was prepared using the formaldehyde method, and cross-verification was employed to ensure the specificity of the polyclonal antibodies. An indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) was successfully established based on the antibodies. The results demonstrated that the established ic-ELISA exhibited a detection range of 12.20 to 2 143.42 ng/mL, with a limit of detection (LOD) at 2.69 ng/mL. This method is simple, rapid, and cost-effective, offering high sensitivity and excellent specificity. It provides a novel option for the efficient detection of furosine in food, demonstrating its potential for practical application.