枸杞多糖体外调节人体肠道菌群的功能研究

Study on the Function of Lycium barbarum Polysaccharides in Regulating Human gut microbiota in vitro

摘要:

为研究枸杞多糖(LBP)对人体肠道菌群结构和代谢产物的影响，对6名健康人的粪便提取物进行单独样本与混合样本的体外模拟厌氧发酵，利用16S rRNA基因高通量测序技术对发酵后肠道菌群进行结构和功能分析，并利用超高效液相色谱(UPLC)检测发酵液中的短链脂肪酸(SCFAs)浓度。结果表明，LBP能够明显改变人体肠道菌群结构与功能，提高肠道菌群中益生菌乳酸杆菌属与双歧杆菌属的丰度，并促进了SCFAs的产生。因此，LBP能够显著影响人体肠道菌群结构与功能。

Abstract:

This study investigated the effects of Lycium barbarum polysaccharide(LBP) on the structure and metabolites of the human gut microbiota. The individual and mixed samples of fecal extracts from six healthy individuals was subjected to anaerobic fermentation in vitro. The structure and function of gut microbiota after LBP fermentation were analyzed by 16S rRNA gene high-throughput sequencing. The concentration of short-chain fatty acids(SCFAs) in the fermentation broth was detected by ultra-high-performance liquid chromatography(UPLC). The results demonstrated that LBP significantly changed the structure and function of human gut microbiota, increasing the abundance of probiotics Lactobacillus and Bifidobacterium in gut microbiota while promoting the production of SCFAs. Consequently, LBP can markedly modulate the structure and function of human gut microbiota.