吡哆醇对鲁氏接合酵母高盐胁迫耐受的影响

Effect of Pyridoxine on Salt Tolerance in Zygosaccharomyces rouxii

摘要:

为了提高鲁氏接合酵母抗高盐胁迫能力，作者探索了添加吡哆醇对鲁氏接合酵母高盐胁迫耐受的影响。通过测量甘油、海藻糖含量以及Na⁺/K⁺-ATP酶活性等一系列生理表征来验证鲁氏接合酵母高盐适应性的变化。实验结果表明，鲁氏接合酵母在高盐胁迫条件下外源添加吡哆醇，在延滞期时，菌株提前24 h积累细胞内甘油，并且加快了细胞内海藻糖分解代谢速率；在对数期时，细胞内钠钾比率（Na⁺/K⁺）降低82.3%，细胞膜上Na⁺/K⁺-ATP酶活性增加16.9%，2-苯乙醇产量（质量浓度）提高6.42倍；在稳定期时，生物量提高10.6%，乙醇产量（质量浓度）提高5%，2-苯乙醇产量（质量浓度）提高1.26倍。综上所述，吡哆醇的添加能有效提高鲁氏接合酵母的高盐胁迫耐受能力，进一步增强了鲁氏接合酵母在酱油等高盐发酵食品中的应用前景。

Abstract:

To improve the salt tolerance of Zygosaccharomyces rouxii (Z. rouxii), the effect of pyridoxine on the tolerance to high salt stress of Z. rouxii was explored. A series of physiological characterization tests, including measurements of glycerol, trehalose levels and Na⁺/K^+-ATPase activity, were carried out to verify the changes of high-salt adaptability of Z. rouxii. The experimental results indicated that yeast cells during the lag phase under high salt stress accumulated intracellular glycerol 24 hours in advance and accelerate the catabolism rate of intracellular trehalose by addition of pyridoxine. In the mid-logarithmic growth phase, the ratio of intracellular Na⁺/+ decreased by 82.3%, while the activity of Na⁺/K⁺-ATPase on cell membrane increased by 16.9%. Additionally, the production of 2-phenylethanol (mass concentration) increased by 6.42-fold. In the stable phase, the biomass increased by 10.6%, the ethanol production (mass concentration) increased by 5%, and the 2-phenylethanol production (mass concentration) increased by 1.26-fold. In conclusion, the addition of pyridoxine could effectively improve the high salt stress tolerance of Z. rouxii, and further enhance the application prospect of Z. rouxii in high-salt fermented foods such as soy sauce.