Abstract:This study investigated the effect of subculture of species on the viability of extracellular enzymes and the morphology, yield and quality of Ganoderma lucidum fruiting bodies during cultivation. The effects of subculture of species on the viability of extracellular enzymes, the biological efficiency of dry basis of fruiting bodies, and the contents of leachables, polysaccharides, triterpenes and sterols, and components of G. lucidum acid were investigated by colorimetry and HPLC. Correlation analysis was performed to explore the correlation between extracellular enzyme activity and quality of G. lucidum. The investigation showed that the activities of G. laccase, filter paper enzyme, cellulase and amylase showed a decreasing trend with the increase of subculture number of species. Correlation analysis showed that laccase, filter paper enzyme, cellulase and amylase activities were positively correlated with fruiting body dry based biological efficiency and the contents of leachables, polysaccharides, ganoderic acid A, ganoderic acid D, ganoderic acid F, ganoderenic acid A and ganoderenic acid D. Further pathway analysis identified the filter paper enzyme was the key extracellular enzyme affecting the biological efficiency of G. lucidum stem-based substrates, the content of polysaccharide and ganoderic acid F, and cellulase was the key extracellular enzyme affecting the content of ganoderenic acid A. Therefore, the subculture number of G. lucidum species should be controlled within 5 generations in practical production, so that the main extracellular enzymes in the matrix can maintain high viability, and the G. lucidum stem-based biology is of high efficiency and quality.
