复合除油微生物菌剂的油脂去除特性

Study on the Grease Removal Characteristics of the Compound Degreasing Microbial Agent

摘要:

为扩大油脂降解的菌种库资源，提高餐厨垃圾中油脂的生物转化效率，作者以所在实验室自行保藏的4株微生物菌株为油脂降解菌，通过等比例复配获得研究用复合除油微生物菌剂，研究了影响菌剂除油效果的因素及组成菌株产脂肪酶的能力。结果表明，复合除油微生物菌剂在温度为25~55 ℃、pH为4~9、盐质量浓度为5~90 g/L、油脂体积分数1%~5%、接种体积分数1%~9%时，均具有一定的除油能力，除油率最高可达84.75%。单一菌株实验结果表明，菌株B501所产脂肪酶的酶活最高，而菌株009所产脂肪酶处于高酶活的持续时间较长，因此除油能力也最强。此外，复合菌剂的除油效果优于单一菌株。

Abstract:

To expand the strain pool of oil degradation and to improve the biotransformation efficiency of oil in food waste, 4 microbial strains preserved in the laboratory were used as lipase degrading bacteria, the compound oil-removing microbial agent was obtained by equal proportion mixture of the 4 strains. The factors affecting oil removal and lipase production ability of the different strains were investigated. The results showed that the compound oil-removing microbial agent had oil-removing ability at temperature 25~55 ℃, pH 4~9, salt content 5~90 g/L, oil concentration 1%~5%, inoculum 1%~9%, and the degradation rate of oil can reach up to 84.75%. The results of the single strain experiments showed that the strain B501 had the highest lipase activity, while strain 009 had the strongest oil removal ability because of its longer duration of high lipase activity. Also, the oil removal effect of compound bacteria is better than that of a single strain.