Abstract:This study evaluated the safety of Pediococcus pentosaceus 10-a-1 with high resistance and adsorption properties to heavy metal lead, which was isolated and screened from sheep manure in Baotou Iron and Steel Mining Area, Baotou City, Inner Mongolia Autonomous Region. Clean grade Kunming mice were selected and administered with high, medium, and low doses of Pediococcus pentosaceus 10-a-1 by oral gavage. The negative control group was administered with the same amount of 0.85 g/dL physiological saline by oral gavage, and the positive control group was administered with 40 mg/kg cyclophosphamide solution by oral gavage. The changes in physical signs, weight gain and food intake of mice were observed during the experiment. Furthermore, the changes of serum biochemical indexes, sperm malformation rate, bone marrow cell malformation rate, organ index and intestinal mucosa morphology were compared among the groups. The results showed that in the acute toxicity test, the mice showed normal signs and no bacterial translocation occurred in the internal organs. In the mouse sperm abnormality test and erythrocyte micronucleus test, the mutation effect of the positive control group was significant, while no significant difference was observed between the Pediococcus pentosaceus 10-a-1 dose groups and the physiological saline control group (P>0.05). In the 28-day toxicity test, there was no significant difference between the Pediococcus pentosaceus 10-a-1 dose groups and the physiological saline group in terms of reproductive performance, organ index, serum biochemical indexes and intestinal mucosal thickness P>0.05). Collectively, Pediococcus pentosaceus 10-a-1 is discovered to be safe without toxic side effects in use.
