食物黄酮异荭草素及其银纳米颗粒的保肝活性

Hepatoprotective Effect of Isoorientin and Its Silver Nanoparticle

摘要:

为提高食物黄酮异荭草素（Iso）的生物活性和预防苯并（a）芘（BaP）诱发的肝损伤提供一定的依据，以Iso及其银纳米颗粒（Iso-AgNPs）为研究对象，建立食品高温有害产物BaP损伤HL-7702人肝细胞模型，基于细胞凋亡、自噬和焦亡3种程序性死亡方式，通过MTT、台盼蓝染色、荧光标记、流式细胞术、Western blotting等方法检测Iso-AgNPs的保肝作用。结果表明：相较于Iso，Iso-AgNPs能更好地钝化BaP对HL-7702人肝细胞活力的抑制作用，促进细胞增殖，显著抑制BaP诱导的HL-7702人肝细胞凋亡性、自噬性和焦亡性损伤。因此，Iso-AgNPs可提高Iso的生物活性且预防肝细胞损伤。

Abstract:

To improve the biological activity of food flavone isoorientin (Iso) and provide a basis for preventing liver injury induced by benzo (a) pyrene (BaP), Iso and its silver nanoparticles (Iso-AgNPs) were used to investigate to the damage of HL-7702 human liver cells caused by BaP, a harmful product of food prepared under high temperature. The hepatoprotective effect of Iso-AgNPs was detected by MTT, trypan blue staining, fluorescence labeling, flow cytometry, and Western blotting based on the three programmed death modes including apoptosis, autophagy and pyroptosis. The results showed that compared with Iso, Iso-AgNPs could better inhibit the damage of HL-7702 huamn liver cells activity induced by BaP, promote cell proliferation, and significantly inhibit the apoptosis, autophagy and apoptosis of HL-7702 huamn liver cells. Therefore, Iso-AgNPs could improve the biological activity of Iso and prevent liver cell damage.