绿原酸对UVB损伤人永生化表皮角质细胞的保护作用

Protective Effect of Chlorogenic Acid on UVB Damaged HaCaT Cells

摘要:

为研究绿原酸（chlorogenic acid，CGA）对UVB损伤人永生化表皮角质细胞（HaCaT）的保护作用及机制，通过MTT法建立细胞紫外损伤（光老化）模型及绿原酸的处理方式，采用酶标仪检测绿原酸的紫外吸收，流式细胞术检测细胞的凋亡水平，ABTS法检测绿原酸的自由基清除能力。结果表明：UVB辐射剂量为21.6 mJ/cm²，绿原酸浓度为100.000、150.000 μmol/L，预处理1 h后细胞培养12 h，绿原酸在UVB波段（280~320 nm）内有较强的吸收；100.000 μmol/L和150.000 μmol/L 绿原酸均能显著降低UVB引起的细胞凋亡；150.000 μmol/L 绿原酸能有效增加UVB处理细胞的ABTS⁺自由基清除能力，达到了53%。由此可知，绿原酸在UVB诱导的HaCaT光老化模型上，具有很强的抗皮肤光老化活性，其机制可能与绿原酸的紫外吸收以及抗氧化能力相关，为富含绿原酸的食品资源开发利用提供科学依据。

Abstract:

To investigate the protective effect and mechanism of chlorogenic acid (CGA) on UVB damaged human immortalized epidermal cells (HaCaT), a cellular ultraviolet damage (photo-aging) model and different treatment methods of CGA were established using MTT assay. The UV absorption of CGA was detected by the microplate reader. The level of apoptotic cell death was determined by flow cytometry analysis. The free radical scavenging ability of CGA was detected by ABTS method. The results showed that when the UVB radiation dose was 21.6 mJ/cm² and the concentrations of CGA were 100.000 and 150.000 μmol/L respectively, after pre-treatment for 1 hour and cell cultivation for 12 hours, CGA exhibited strong absorption in the UVB spectrum range of 280~320 nm. Both 100.000 μmol/L and 150.000 μmol/L CGA could significantly reduce UVB-induced cell apoptosis. The ABTS⁺ free radical scavenging capacity of UVB-treated cells could be effectively increased by 150.000 μmol/L CGA, reaching 53%. Therefore, CGA has strong anti-skin aging activity in the UVB-induced HaCaT photo-aging model, which might be related to its UV absorption and antioxidant ability. This provides a scientific basis for the development and utilization of food resources rich in CGA.