Abstract:The frozen Takifugu obscurus is delicious, but there are no products related to room-temperature fish soup in the market. In order to solve the problems of instability of fish soup such as flocculation and stratification during storage and to prolong its storage period, this study investigated the effects of homogenization conditions on the stability of Takifugu obscurus soup based on the average particle size, dispersion coefficient and ζ-potential of micro- and nano-particles in fish soup, and the effects of different sterilization methods on the nutrition and flavor quality of fish soup were studied. The results showed that the best stability of fish soup was achieved when homogenized under 30 MPa at 60 ℃ for 2 times. By comparing different sterilization methods, the loss of nutrients in the ultra-high temperature sterilization group was smaller than that in the pasteurized group or in the thermal temperature sterilization group. In addition, the contents of total nitrogen, amino acid nitrogen and collagen in the fish soup decreased by 0.92%, 2.73% and 4.38%, respectively, compared with the control group. The flavor components were most similar to those in the control group, and only trans-2-octenal as a characteristic flavor component increased. The pasteurized group had low cost, however, its storage time was only 6 weeks. The storage time of fish soup in the thermal sterilization group was up to 20 weeks, but the relative content of the compounds expressing the steaming flavor increased, which had an adverse effect on the flavor of fish soup. This study could provide theoretical reference and data support for the industrial production of puffer fish soup.