打叶复烤烟叶逸出物的成分分析及VC对其诱导的EA.hy926细胞损伤的保护作用

Components in Exudates from Threshing and Redrying Tobacco Leaves and Protective Effect of VC on EA.hy926 Cell Damage Induced by the Exudates

摘要:

【目的】探索打叶复烤烟叶逸出物的成分及其对人血管内皮（EA.hy926）细胞损伤的影响。【方法】用打叶复烤烟叶逸出物建立EA.hy926细胞毒性损伤模型，经VC预处理后，采用四甲基偶氮唑蓝（MTT）法评估EA.hy926细胞存活率，并检测EA.hy926细胞内活性氧（ROS）相对含量。【结果】打叶复烤烟叶逸出物刺激24 h可造成明显的细胞损伤，成功建立血管内皮细胞毒性损伤模型，VC预处理可以明显逆转上述变化。【结论】VC对打叶复烤烟叶逸出物诱导的EA.hy926细胞毒性损伤有明显的保护作用，其作用机制与降低细胞内活性氧水平相关。

Abstract:

[Objective] This study aimed to explore the components in the exudates from threshing and redrying tobacco leaves and their effects on human vascular endothelial (EA.hy926) cell damage. [Method] The EA.hy926 cell model of cytotoxic damage was established with the exudates from threshing and redrying tobacco leaves. After pretreatment with VC, the methyl thiazolyl tetrazolium (MTT) method was employed to evaluate the viability of EA.hy926 cells, and the relative content of reactive oxygen species (ROS) in EA.hy926 cells was determined. [Result] Obvious cell damage was observed after 24 h of stimulation with the exudates from threshing and redrying tobacco leaves, which indicated the cell model of cytotoxic damage was successfully established. VC pretreatment significantly reversed the above changes. [Conclusion] VC exerts obvious protective effect on the cytotoxic damage of EA.hy926 cells that is induced by the exudates from threshing and redrying tobacco leaves by reducing the ROS level.