动物双歧杆菌乳亚种对便秘的改善研究

Exploration of the Effect of Bifidobacterium animalis subsp. lactis on Improving Constipation

摘要:

便秘是常见胃肠道疾病，部分动物双歧杆菌乳亚种（Bifidobacterium animalis subsp. lactis， B. lactis）已被证明对便秘有改善作用，但效果存在差异。为研究不同动物双歧杆菌乳亚种菌株对便秘的缓解效果，建立便秘动物模型，通过3株动物双歧杆菌乳亚种菌株的干预试验，测定便秘相关指标、肠道菌群及其代谢产物水平，探究各菌株缓解便秘的作用及机制。结果表明，CCFM1278和BB12干预显著改善便秘小鼠排便质量、排便粒数、粪便含水质量分数、首粒黑便时间和小肠推进率，而FJSSZ6M5的干预效果不显著。CCFM1278干预调节便秘小鼠肠道菌群中Akkermansia、Ruminiclostridium 6、Ruminococcus 1和Alloprevotella相对丰度，改善短链脂肪酸和脱氧胆酸水平，促进胃泌素、生长抑素分泌，抑制水通道蛋白3和水通道蛋白8的基因转录水平的表达。综上，动物双歧杆菌乳亚种CCFM1278具有缓解便秘的潜力，可将为便秘患者的治疗提供新方向。

Abstract:

Constipation is a common gastrointestinal disorder. Some Bifidobacterium animalis subsp. lactis has been shown to have an ameliorative effect on constipation, but there is variation in their effectiveness. In order to investigate the effect of different B. lactis strains on constipation, an animal model of constipation was established, and the constipation-related indexes, gut microbiota and their metabolite levels were determined through the intervention experiments of three B. lactis strains, to investigate the effects of constipation alleviation of the strains and their mechanisms. The results showed that CCFM1278 and BB12 significantly improved the weight of defecation, number of fecal pellets, fecal water content, time to first black stool, and small intestinal propulsion rate in constipated mice, while the effect of FJSSZ6M5 was not significant. B. lactis CCFM1278 modulated the relative abundance of Akkermansia, Ruminiclostridium 6, Ruminococcus 1 and Alloprevotella in the gut microbiota of constipated mice, improved the abnormal levels of short-chain fatty acids and deoxycholic acid, significantly improved the secretion of gastrin and somatostatin and inhibited the expression of aquaporin 3 and aquaporin 8. In conclusion, B. lactis CCFM1278 has the potential to relieve constipation. This study provides a new direction for the treatment of patients with constipation.