啶虫脒分子印迹荧光传感器的构建及检测研究

Fabrication and Detection of Molecularly Imprinted Fluorescence Sensor Based on Acetamiprid

摘要:

作者首先修饰和制备了对啶虫脒农药有特异响应的硅碳量子点（SiCDs），并以此作为荧光探针开发了可快速检测啶虫脒农药的高灵敏分子印迹荧光传感器SiCDs@MIPs，同时研究了模板与功能单体物质的量比、交联剂添加量、SiCDs包埋量对SiCDs@MIPs检测灵敏度的影响。结果表明，SiCDs@MIPs的最适制备条件为：模板与功能单体的物质的量比为1∶4，交联剂添加量为25 μL，SiCDs包埋量为10 mg。在最优检测条件下，SiCDs@MIPs对啶虫脒的检出限为0.086 μmol/L，线性范围为0.045~0.450 μmol/L，相关系数为0.989，检测时间仅需25 min。进一步将制备的SiCDs@MIPs应用于白菜、番茄和冬瓜中啶虫脒农药残留的检测，回收率可达到73.0%~109.0%。该方法为蔬菜中啶虫脒农药残留的检测提供了新策略。

Abstract:

In this study, the silicon carbon quantum dots (SiCDs) with a specific response to acetamiprid pesticides were modified and prepared, which were then used as the fluorescence probe to develop a highly sensitive molecularly imprintied fluorescence sensor (SiCDs@MIPs) to rapidly detect the acetamiprid pesticides. The effects of the ratio of template to functional monomer, the amount of cross-linking agent, and the amount of SiCDs on the detection sensitivity of SiCDs@MIPs were further investigated. The results showed that the optimal preparation conditions of SiCDs@MIPs were as follows: the ratio of template to functional monomer was 1∶4, the amount of cross-linking agent was 25 μL, and the embedding amount of SiCDs was 10 mg. Under the optimal detection conditions, the detection limit of SiCDs@MIPs toward acetamiprid was 0.086 μmol/L within the linear range of 0.045 to 0.450 μmol/L, the correlation coefficient was 0.989, and the detection time was only 25 minutes. When SiCDs@MIPs was further applied to determine the acetamiprid pesticide residues in Chinese cabbage, tomato and wax gourd, the recoveries ranged from 73.0% to 109.0%. The proposed method provided a new strategy for the determination of acetamiprid pesticide residues in vegetables.