植物乳杆菌CCFM8661增强小鼠免疫力的功能评价

Functional Evaluation of Lactiplantibacillus plantarum CCFM8661 for Enhancing Immunity in Mice

摘要:

为了通过动物实验评价植物乳杆菌（Lactiplantibacillus plantarum） CCFM8661的免疫调节功能,作者采用120只雄性BALB/c小鼠，分别灌胃植物乳杆菌CCFM8661（低、中、高3个剂量）或安慰剂30 d。之后采用以下方法综合评估植物乳杆菌CCFM8661调节小鼠免疫力的功效：小鼠脾淋巴细胞转化实验及迟发型变态反应（delayed type hypersensitivity, DTH）实验评价小鼠细胞免疫功能；血清溶血素生成实验及抗体生成细胞检测实验评价小鼠体液免疫功能；碳廓清实验及腹腔巨噬细胞吞噬荧光微球实验评价小鼠单核-巨噬细胞功能；自然杀伤（nature-killer, NK）细胞活性检测评估小鼠NK细胞功能。结果显示，植物乳杆菌CCFM8661可明显增强小鼠迟发型变态反应的耳肿胀程度、提升小鼠脾淋巴细胞的增殖水平、促进小鼠血清溶血素的生成、提高小鼠腹腔巨噬细胞的吞噬能力及机体碳处理能力。研究结果表明植物乳杆菌CCFM8661具有增强小鼠免疫力的作用，为其实际应用提供理论依据。

Abstract:

This study aims to evaluate the immunomodulatory function of Lactiplantibacillus plantarum (L. plantarum) CCFM8661 through animal experiments. A total of 120 male BALB/c mice were administrated with low, medium, and high doses of L. plantarum CCFM8661 or placebo by gavage for 30 d. The immunomodulatory capacity of L. plantarum CCFM8661 in mice was comprehensively evaluated by a series of experiments, including mouse splenic lymphocyte transformation and delayed type hypersensitivity (DTH) experiments to evaluate cellular immune function of the mice, serum hemolysin production assay and antibody-producing cell detection assay to assess humoral immune function of the mice, carbon clearance test and peritoneal macrophage swallowing fluorescent microspheres test to evaluate the monocyte-macrophage function of the mice, and natural killer (NK) cell activity assessment to evaluate the NK cell function of the mice. The results showed that L. plantarum CCFM8661 significantly enhanced the degree of ear swelling in the DTH response, increased the proliferation level of mouse spleen lymphocytes, promoted the production of serum hemolysin, and improved the phagocytosis capacity of peritoneal macrophages and carbon clearance capacity in the mice. These results indicated the capacity of L. plantarum CCFM8661 to enhance the immunity of mice, providing a theoretical basis for its practical application.