不同产地黄观音乌龙茶的广泛靶向代谢组学分析

Widely Targeted Metabolomic Analysis of Huang Guanyin Oolong Tea from Different Areas

摘要:

为探究不同产地黄观音乌龙茶品质的差异，采用广泛靶向代谢组学及茶叶感官审评的方法，对云霄和武夷山生产的黄观音乌龙茶代谢物进行分析。结果表明，两地黄观音乌龙茶共测出11类820种代谢物，其中两地一级黄观音乌龙茶筛选出的差异代谢成分63种，占总代谢物的7.68%；两地二级黄观音乌龙茶鉴定出的差异代谢成分120种，占总代谢物的14.63%。KEGG代谢通路分析表明，两地一级黄观音乌龙茶富集程度最高的通路中有3条与黄酮类差异代谢物相关，两地二级黄观音乌龙茶富集程度最高的通路中有2条与氨基酸及其衍生物差异代谢物相关。通过对比不同产地黄观音乌龙茶的呈味物质发现，两地一级黄观音的槲皮素-3-O-桑布双糖苷、山柰酚-3-O-鼠李糖苷、芹菜素-7-O-芸香糖苷、L-脯氨酸、L-天冬酰胺和γ-氨基丁酸等代谢物含量的差异达到极显著水平；而两地二级黄观音的L-谷氨酸、L-天冬酰胺和γ-氨基丁酸等代谢物含量的差异达到极显著水平。该研究利用广泛靶向代谢组学技术，筛选出与云霄黄观音和武夷黄观音乌龙茶品质相关的差异代谢物，为阐明两地黄观音乌龙茶的品质特征提供参考。

Abstract:

The metabolites of Huang Guanyin oolong tea produced in Yunxiao and Wuyishan were analyzed by widely targeted metabolomics and sensory evaluation to investigate the quality differences of Huang Guanyin oolong tea from different areas. The results showed that a total of 820 metabolites from 11 classes were identified in Huang Guanyin oolong tea from the two areas. The different metabolic components identified from the first-grade oolong tea were 63, and 120 for the second-grade oolong tea, accounting for 7.68% and 14.63% of the total metabolites, respectively. The KEGG metabolic pathway analysis showed that three highest enrichment pathways of the first-grade Huang Guanyin oolong teas from two areas were related to the flavonoid differential metabolites, and two highest enrichment pathways of the second-grade Huang Guanyin oolong teas from two areas were related to the differential metabolites of amino acids and their derivatives. According to the comparison of flavor components in Huang Guanyin oolong teas from different areas, the content of differential metabolites including quercetin-3-O-sambubioside, kaempferol-3-O-rhamnoside, apigenin-7-O-rutinoside, L-proline, L-asparagine and γ-aminobutyric acid in the first-grade Huang Guanyin oolong tea from two areas were extremely significant, and the content difference of metabolites including L-asparagine, L-glutamic and γ-aminobutyric acid in the second-grade Huang Guanyin oolong tea from two areas were extremely significant. In this study, the differential metabolites associated with the quality of Yunxiao and Wuyi Huang Guanyin oolong teas were screened out based on the widely targeted metabolomics analysis, providing a reference for elucidating the quality characteristics of Huang Guanyin oolong teas from the two areas.