酿酒酵母代谢调控合成功能营养品：进展与挑战

Metabolic Regulation and Synthesis of Functional Nutrients in Saccharomyces cerevisiae: Progress and Challenges

摘要:

综述了酿酒酵母作为底盘细胞代谢调控合成功能营养品的研究进展。首先，介绍了酿酒酵母作为细胞工厂相关的启动子调控、终止子调控、辅因子调控、转运蛋白调控及动态调控等一系列代谢调控策略，并介绍了加强产物合成的相关遗传改造工具和代谢分析技术。随后，概述了酿酒酵母合成寡糖、维生素及萜类、短链有机酸和脂肪酸等功能营养品的研究进展。最后，提出了目前面临的挑战，并结合代谢工程新兴技术展望了未来酿酒酵母代谢调控的发展趋势，以期为功能营养品在酿酒酵母中的高效合成提供参考。

Abstract:

The research progress of Saccharomyces cerevisiae as a chassis cell for metabolic regulation and synthesis of functional nutrients was summarized in this review. Firstly, a series of metabolic regulation strategies related to the promoter regulation, terminator regulation, cofactor regulation, transporter regulation and dynamic regulation of Saccharomyces cerevisiae as a cell factory were introduced, and the related genetic transformation tools and metabolic analysis techniques to enhance product synthesis were also introduced. Secondly, the research progress on synthesis of functional nutrients, such as oligosaccharides, vitamins and terpenoids, short chain organic acids and fatty acids, by Saccharomyces cerevisiae was summarized. Lastly, the current challenges were put forward, and the future development trend of metabolic regulation of Saccharomyces cerevisiae was prospected in combination with the emerging technologies of metabolic engineering, in order to provide a reference for the efficient synthesis of functional nutrients in Saccharomyces cerevisiae.