蜂王浆中活性因子对衰老小鼠的保护作用

Protective Effects of the Active Fractions of Royal Jelly on the Aging Mice

摘要:

为了探索蜂王浆及其主要的蛋白质类、脂类功能活性因子对D-半乳糖（D-gal）致衰老小鼠的保护作用。实验通过透析、超滤、有机相萃取的方法分别提取分离了蜂王浆中粗蛋白、蛋白质类功能因子及脂类功能因子。通过连续7周每日腹腔注射100 mg/kg（以干质量计） 的D-gal建立衰老小鼠模型，并分别给予蜂王浆及不同剂量的粗蛋白组分、蛋白质类或脂类功能因子干预后，发现蜂王浆可以显著提高小鼠在T-迷宫中的准确率，并且降低肝脏器官指数，证实蜂王浆可能提高衰老小鼠的认知记忆水平，改善小鼠的衰老症状；脂类功能因子及高剂量的蛋白质类功能因子能够显著提高 T-迷宫准确率、缓解肝脏肿大。因此推测，蜂王浆中的蛋白质类功能因子和脂类功能因子都具有良好的抗衰老作用，其机制可能与提高大脑的记忆能力有关。

Abstract:

This study explored the protective effects of royal jelly (RJ) and its major protein and lipid fractions on the D-galactose (D-gal) induced aging mice. In the experiment, the crude protein fractions, functional protein and lipid fractions were extracted and separated from royal jelly by dialysis, ultrafiltration and organic solvents, respectively. The aging mice model was established by intraperitoneal injection of 100 mg/kg D-gal per day for 7 weeks. The improved precision rate of mice in the T-maze and the reduction of liver organ index in mice intervened by royal jelly proved its beneficial functions on the aging-associated impairments of cognition and memory. Moreover, intervention of functional lipid fractions and functional protein fractions under high dose could significantly improve the accuracy of mice in T-maze and relief the liver enlargement. Therefore, both the functional protein and lipid fractions of royal jelly performed desirable anti-aging effects, and the mechanism may be related to the improvement of memory capacities.